Fig. 6.

Cell proliferation during half-body regeneration. A Schematic of the EdU incorporation experiment in cydippids bisected through the oral-aboral axis. B Box plot showing the levels of cell proliferation at the wound site at different time points after bisection. The thick horizontal bars indicate the median values. Each dot represents one individual. C–F’ Confocal stack projections of bisected cydippids through the oral-aboral axis oriented in a lateral view showing the cut site in the first plane. The time following bisection is listed to the left of the rows. Nuclei of S-phase cells are labeled with EdU (magenta), and all nuclei are counterstained with DAPI (blue). The pattern of EdU labeling corresponding to each time point is shown in a cartoon on the right of the rows. Dotted line rectangles in C, D, and E show the area corresponding to higher magnifications on the right. White dotted lines in E” and F’ delimit the area corresponding to the regenerating comb rows and tentacle bulb. White asterisks point to tentacle bulbs of the uncut site. Note that EdU+ cells at 72 hpa (F’) are located at the oral end of the regenerating comb rows and no EdU+ cells are detected at the aboral end where cells are already differentiated. Scale bars = 100 μm. Abbreviations: hab hours after bisection, cr comb row, tb tentacle bulb