Fig. 7.

Cell proliferation during apical organ regeneration. A Schematic of the EdU incorporation experiment in cydippids in which the apical organ was amputated. B Box plot showing the levels of cell proliferation at the wound site at different time points post amputation. The thick horizontal bars indicate the median values. Each dot represents one individual. C–H” Confocal stack projections of cydippids in which the apical organ was amputated at different time points post amputation. Aboral and lateral views are shown. The labeling corresponding to each panel is listed at the top of the columns, and the time following amputation is listed to the left of the rows. Dotted line rectangles in C, D, E, F, G, and H show the area corresponding to higher magnifications on the right. White rectangles in C” and D” delimit the area of apical organ regeneration. Nuclei of S-phase cells are labeled with EdU (magenta), and all nuclei are counterstained with DAPI (blue). DIC images of the tissue are shown in F’ and H’. Scale bars = 100 μm. Abbreviations: tb tentacle bulb, ao fl apical organ floor, hpa hours post amputation