Fig. 10
From: GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse
GLIPR1L1 loss disrupts IZUMO1 redistribution during the acrosome reaction. a Sperm were stained for IZUMO1 localization and the acrosome was labeled with PNA (green), while DNA was stained with DAPI (blue). Scale bar = 20 μm. b The percentage of sperm that displayed IZUMO1 relocalization was scored in non-acrosome-reacted (Non-AR) and acrosome-reacted (AR) sperm from WT and Glipr1l1−/− mice. This experiment was replicated five times with a minimum of 200 spermatozoa being examined in each. Representative images are shown. *P < 0.05, **P < 0.01, ***P < 0.001. Individual data points for each replicate are provided in Additional file 6: Raw data