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Fig. 5 | BMC Biology

Fig. 5

From: GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse

Fig. 5

Co-localization of IZUMO1 and GLIPR1L1 in developing mouse germ cells using an in situ proximity ligation assay. a The cells were counterstained with DAPI (blue) and PNA (green). This experiment was replicated three times, and representative images are shown. b In the case of capacitated spermatozoa, a lower magnification image is also included to highlight the differences in PLA labeling between acrosome intact and acrosome-reacted (arrowhead) spermatozoa. Scale bar = 10 μm. c The percentage of cells displaying PLA positive labeling was recorded. Each experiment was replicated three times and the data are expressed as the mean ± S.E.M. *P < 0.05, compared with spermatocytes. Individual data points for each replicate are provided in Additional file 6: Raw data

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