Fig. 7
From: GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse
Glipr1l1 expression and immunofluorescent localization in mouse sperm from wild type and Glipr1l1−/− mice. a qPCR analysis of Glipr1l1 mRNA levels in isolated testis and germ cells from Glipr1l1−/− mice relative to wild type (WT) mice. mRNA expression levels were normalized to the housekeeping gene Ppia. This experiment was replicated three times and data is shown as mean ± S.D., **** P < 0.0001. Individual data points for each replicate are provided in Additional file 6: Raw data. b Localization of GLIPR1L1 at the sperm head and at the connecting piece. GLIPR1L1 staining (red, marked with white arrows) was observed in WT sperm and no staining was observed in the Glipr1l1−/− sperm. In all the images, nuclear DNA was stained with DAPI (blue). This experiment was replicated three times and representative images are shown. Scale bar = 20 μm