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Fig. 3 | BMC Biology

Fig. 3

From: From spiral cleavage to bilateral symmetry: the developmental cell lineage of the annelid brain

Fig. 3

Differentiated cell types with known lineage at 30 hpf. A, B Cell types identified directly in the last frame of the time-lapse recordings by their position and morphological features. The larval eye pigment cells identified by their autofluorescence in the red spectrum (A, white arrows). The large gland cells with the typical flask shape and large size (A, white arrowheads). B Several cell types of the apical organ could be identified by their morphology and position (described in detail in [14]). C The WMISH of chat performed on the live-imaged larva (shown in panel A) fixed immediately after imaging allows addressing the cell lineages of cholinergic neurons. Yellow arrows indicate the position of larval eye photoreceptors, orange arrowheads the ventrolateral ChAT+ cells, red arrowhead the apical ChAT+ cells with first lateral axons, and blue arrowheads the ventromedial cholinergic cells. D Two apical neurons with axons revealed by injection of the AB blastomere with la-egfp mRNA. The neuron with the ventrolateral projections lies outside from the rest of the AB-labeled domain (D’ and D”). E, E’ The snapshots of a time-lapse recording of larvae injected with h2a-rfpmRNA at 1-cell stage and la-egfp to the D blastomere shows the axon of a flask-shaped cell in the apical organ (yellow arrowhead) and the growing axon of the ventral cholinergic cells (yellow arrow). The prototroch ring is indicated by a dashed crescent. F A summary diagram of the differentiated cell types in the episphere at ~ 30 hpf. The numbering corresponds to the first column of Table 1

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