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Fig. 6 | BMC Biology

Fig. 6

From: A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

Fig. 6

Exosomes and ER exhibit specific cellular labeling via the minimal GCE-tag in the presence of TAMRA-Tet. COS7 cells were transfected with the indicated plasmids, labeled when indicated, and imaged live 48 h later. a, b, d, e Left to right: maximum projection images, zoomed-in images of a subset of the cells (correspond to squares in left panels), line intensity profiles measured for the dashed lines in middle panels. c, f SNR values measured in cells expressing the indicated plasmids (n Exo70 = 40, ERcb5TM = 45). g Cells were transfected with the indicated the pBUD-Pyl-RS plasmids and labeled with SiR-Tet or TAMRA-Tet, as indicated. For each condition, mean intensity levels were measured for a region of interest inside the cell and were divided by the mean background intensity measured outside the cell. pBUD-Pyl-RS plasmids that do not encode for a protein of interest were used as negative controls (no protein). n = 15. Results presented in each panel were obtained in at least 3 independent experiments. Scale-bars, 10 μm; zoomed-in images, 2 μm

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