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Fig. 5 | BMC Biology

Fig. 5

From: Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae

Fig. 5

Varied response to temperature of uORF translation. a, b Responses of uORF translation to growth temperature grouped by start codons triplets. The temperature-regulated uORFs [see Fig. 4a, b; highlighted in red or blue in (iii)] were binned according to their start site. a Analysis done for changes observed at 20 °C relative to 30 °C. b Analysis done for changes observed at 37 °C relative to 30 °C. c, d Notched boxplot analysis for changes in TEuORF (ΔTEuORF) on all the uORFs starting with each near-cognate start codon triplet. All uORFs indicates all translated uORFs identified and analyzed in this study (N = 1359); uAUGs (N = 136); first base change (N = 631); second base change (N = 109); and third base change (N = 483). The dotted horizontal line shows the median for All uORFs. c Analysis done for ΔTEuORF at 20 °C. d Analysis done for ΔTEuORF at 37 °C. e, f Scatterplots of ΔTEuORF at 20 °C (x-axes) versus ΔTEuORF at 37 °C (y-axes). e The ΔTEuORF values of temperature-regulated uORFs [Fig. 4a, b, (iii)]. Green circles represent AUG uORFs (uAUGs, N = 14), and pink circles represent NCC uORFs (NCCs, N = 98). The plot is broken into four quadrants, Q1–4, depending on the direction of ΔTEuORF at each temperature. The numbers in parenthesis represent the number of uORFs present in that specific quadrant. The red circle shows the main ORF of F-LucUUG reporter mRNA. f Same as in e, except all translated uORFs identified in this study whose changes in TE could be determined are shown. AUG uORFs (N = 136, green circles) and NCC uORFs (N = 1223, pink circles)

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