Fig. 1

The supplementation of nutrients elevated fat storage and the expression of CPL-1 in C. elegans. a Schematic representation of glucose or palmitic acid supplementation. b Representative images and quantification of Oil Red O staining in N2 worms induced by the supplementation of glucose (at 1 mM and 5 mM) or palmitic acid (at 0.02 mM and 0.2 mM). For Oil Red O staining quantifications, the data were obtained from 3 independent experiments and 3000 stained worms were used in each experiment. c Representative image of thin-layer chromatography (TLC) and triglyceride (TAG) contents in N2 worms induced by the supplementation of glucose or palmitic acid, n = 3 independent growths. d Representative images and quantification of GFP fluorescence in DHS-3::GFP worms induced by the supplementation of glucose or palmitic acid. For GFP fluorescence quantification, the data were obtained from 3 independent experiments and 30 worms were imaged and qualified with the level of fluorescence intensity. e Immunoblot and quantification of CPL-1 protein in N2 worms induced by the supplementation of glucose or palmitic acid. The band of mature protein at 25 kD, the active form of CPL-1, was counted as the quantification of CPL-1 to ACT-1, n = 3 independent growths. The data in b–e are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. not significant by one-way ANOVA