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Fig. 5 | BMC Biology

Fig. 5

From: SATB1 establishes ameloblast cell polarity and regulates directional amelogenin secretion for enamel formation

Fig. 5

Deficiency of amelogenin secretion results in a thin and disorganized enamel layer in Satb1−/− mouse. a Amelogenin immunostaining on a sagittal section of the P13 wt mouse incisor shows amelogenins are primarily detected in the enamel matrix and Tomes’ processes (indicated by black triangles). b A representative sagittal section from the P13 Satb1−/− mouse incisor shows intense amelogenin immunoreactive signal in the shortened ameloblasts, no obvious Tomes’ processes, and a thin layer enamel matrix with a comparable intense immunoreactive signal as that of the control enamel (e). Scale bar in a and b, 25 μM. Rab30 was immunolocalized in the cytoplasm closed to the apical surface of wild-type secretory ameloblasts (as indicated by white triangles) (c). More disperse and intense Rab30 immunostaining signal was detected in the cytoplasm, but not at the apical surface (indicated by white triangles) of Satb1−/− mouse secretory ameloblasts (d). The interface between ameloblasts and the enamel matrix was outlined by a white dotted line. Scale bar in panel c and d, 10 μM. Enamel structure in P13 Satb1−/− mouse teeth is disorganized. e Ground sagittal sections of mouse hemimandible were examined under scanning electron microscope at the position highlighted with a red arrow. f Ordered, well-aligned enamel rods were detected in full width of the wt incisor enamel layer. The red dotted line traces one intact enamel rod, which is fabricated by one ameloblast. g No well-defined structure was visualized in the thin layer of enamel of the Satb1−/− mouse at the corresponding position. Scale bar in e, 1 mm; f and g, 2 μm

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