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Fig. 2 | BMC Biology

Fig. 2

From: The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments

Fig. 2

Import of Mpc2 and Mpc3 precursors occurs via the receptor Tom70, not Tom20. Radiolabeled Mpc2 (a) and Mpc3 (b) were imported at 25 °C into mitochondria from wild-type (WT), tom20Δ, or tom70Δ yeast strains and analyzed as described in Fig. 1d. a, b (lower panels) Quantification of import and assembly efficiency; the efficiency into WT mitochondria upon the longest import period was set to 100% (control); n = 3 except Mpc2 import into tom70Δ: n = 4; error bars: SEM. As controls, the matrix-targeted precursor of F1β was imported into tom20Δ mitochondria (c, left panel, with α-Ssc1 immunodecoration as a loading control), and the carrier protein AAC was imported into tom70Δ mitochondria (c, right panel). In all experiments, non-imported precursors were degraded with proteinase K. m, mature form

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