Fig. 2From: The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segmentsImport of Mpc2 and Mpc3 precursors occurs via the receptor Tom70, not Tom20. Radiolabeled Mpc2 (a) and Mpc3 (b) were imported at 25 °C into mitochondria from wild-type (WT), tom20Δ, or tom70Δ yeast strains and analyzed as described in Fig. 1d. a, b (lower panels) Quantification of import and assembly efficiency; the efficiency into WT mitochondria upon the longest import period was set to 100% (control); n = 3 except Mpc2 import into tom70Δ: n = 4; error bars: SEM. As controls, the matrix-targeted precursor of F1β was imported into tom20Δ mitochondria (c, left panel, with α-Ssc1 immunodecoration as a loading control), and the carrier protein AAC was imported into tom70Δ mitochondria (c, right panel). In all experiments, non-imported precursors were degraded with proteinase K. m, mature formBack to article page