Fig. 5
Reduced capacity of β7-F185A T cells to induce chronic colitis. Rag1−/− mice (n = 9 per group) were given 1 × 105 CD4+CD45RBhigh T cells isolated from WT, KI, or KO splenic lymphocytes and monitored for 12 weeks. a Body weight change after T cell transfer. b Quantitative histopathologic grading of colitis severity. c Hematoxylin and eosin staining of colon sections at week 12 after T cell transfer. d Immunofluorescence staining and CD4+ T cell quantification of colon sections with anti-CD4 (red) and DAPI (blue) as in d. Scale bars, 100 μm (c, d). **P < 0.005, ***P < 0.001; ns, not significant (two-way ANOVA in a, Student’s t test in b). Data are mean ± s.d. of at least 3 independent experiments (a, b)