Fig. 2

Pdx mutant phenotype. In situ hybridisation to wild type and Pdx 4Δ homozygous mutant neurulae stage (13 h) amphioxus embryos for Mop (a, b), Mitf (c, d), Pdx (e, f), Cdx (g, h) and Ilp1 (i, j). Embryos were genotyped by PCR after hybridisation. Green fluorescence in 7 gill slit amphioxus larvae showing endodermal patch in wild type (k, k’) but not homozygous Pdx 4Δ mutants (l, l’). Inset k” shows confocal imaging of sibling larva showing fluorescence in endoderm cells. Anterior to the left in all images; dorsal to the top in all cases except a’, b’, c’ and d’ which are dorsal views with the right side to the top of the image. Scale bar in a, 50 μm, refers to a to j; scale bar in k, 200 μm, refers to k to l’