Fig. 1
From: DNA interference is controlled by R-loop length in a type I-F1 CRISPR-Cas system
DNA interference of Aa-CRISPR-Cas system in vivo. a Schematic representation of the Aa-CRISPR-Cas locus composed of six cas genes and CRISPR locus containing 152 spacers. Cascade genes are underlined. Gene names of the previous nomenclature are in parentheses. b Predicted PAM for the Aa-CRISPR-Cas system. WebLogo representation [48] of 5-nt sequences found immediately upstream of phage protospacers that match CRISPR spacers. c Components of Aa-CRISPR-Cas that participate in plasmid or ssDNA phage interference. E. coli cells carrying (i) CRISPR, (ii) CRISPR and Cas1-Cas2/3 (Cas1–2/3), (iii) CRISPR and Cascade, or (iv) complete Aa-CRISPR-Cas system were transformed with pSP-CC plasmid or infected with M13-SP-CC phage that contain matching protospacer and CC PAM. The ratio of transformation or infection efficiencies of non-target and target DNA was expressed as interference. d Promiscuity of PAM in Aa-CRISPR-Cas system. E. coli cells bearing Aa-CRISPR-Cas system were transformed with a set of plasmids containing dinucleotide sequences adjacent to the spacer-matching protospacer and efficiency of transformation (CFU/μg) was assayed for each PAM variant. The plasmid containing a non-matching protospacer was used as a non-targeting control. Error bars in c and d represent standard deviations in at least three separate experiments (individual data values are provided in Additional file 14)