Approach | Source | Key points |
---|---|---|
Genome size estimation | Fig. 2a, Fig S2 | - C. felis from two populations have the same mean genome size. |
- Individual cat fleas vary ~ 118 Mb in estimated genome size. | ||
- Individual rat fleas vary ~ 100 Mb in estimated genome size. | ||
Long-read assembly with proximity ligation | Fig. 1, Fig. S1, Table S5 | - Nine scaffolds > 10 Mb are littered with gene duplications, which comprise 38% of protein-coding genes. |
- No misassembly of allelic variants in the BIG9 scaffolds. | ||
Transcript mapping | Fig. 2d | - 98% of duplicate genes have transcriptional support in RNA-Seq data from an independent colony (1KITE). |
Short-read mapping | Fig. 2c | - Short-read data map with far greater depth to single-copy genes versus duplicate genes. |
Assessment of duplication lengths | Fig. 2e | - 69% of duplications are divergent in length; heterogeneity in length and composition are positively correlated. |