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Fig. 2 | BMC Biology

Fig. 2

From: The evolutionarily conserved ESRE stress response network is activated by ROS and mitochondrial damage

Fig. 2

The combination of rotenone and NAC enhanced ESRE expression. a Fluorescent images of C. elegans strains with 3XESRE::GFP reporter treated with vehicle (DMSO) (left) or 50 μM rotenone (right) with or without 25 mM ascorbate (Asc), 2.5 mM NAC, or ascorbate and NAC combination (NAC/Asc) for 8 h. b Quantification of GFP fluorescence for 3XESRE::GFP reporter after 6-h treatment with rotenone with or without 2.5 mM NAC or 5 mM NAC. c Quantification of GFP fluorescence for a. d Fluorescent images and e quantification of GFP fluorescence of C. elegans carrying Peredox::GFP (NADH/NAD+) reporter treated with vehicle (DMSO), rotenone, or rotenone with 2.5 mM NAC and/or 25 mM ascorbate for 10 h. f Fluorescent images and g quantification of GFP fluorescence of C. elegans with 3XESRE::GFP reporter following treatment with CCCP alone or in combination with 2.5 mM NAC, 25 mM ascorbate, or NAC and ascorbate (NAC/Asc) for 10 h. Representative images are shown. Three biological replicates with ~ 400 worms/replicate were analyzed. Error bars represent SEM. p values were determined from one-way ANOVA, followed by Dunnett’s test. GFP values were normalized to DMSO. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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