Fig. 6
From: Mechanical force-induced morphology changes in a human fungal pathogen
Forces in physical model of PDMS invasive growth. a Forces encountered upon probe displacement into and out of PDMS. A 1-mm-diameter steel probe was advanced perpendicularly into the base of the cylindrical piece of cured PDMS (10:1 PDMS to cross-linker ratio, Young’s modulus ~ 2 MPa). Probe displacement rate was 63 μm/s, and force was determined during different stages, as indicated, i.e., compression, penetration, and after the probe tip emerged from PDMS. Analogous stage of hyphal filament invasive growth indicated in insets along with the growth stage from time-lapse in Fig. 3a (I–V). b, c Resistive forces as a function of PDMS to cross-linker ratio. b Fcrit was determined using a 1-mm-diameter steel probe, with either 3.5- or 8-cm-diameter PDMS cylinders of indicated PDMS to cross-linker ratio, with probe displacement set to 1.6–3.2 μm/s. Bars are SD for on average 10 independent determinations for each PDMS ratio. c Fin was determined by subtracting Fout from Finvas from the probe penetration experiment described in a. Bars are SD