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Fig. 1 | BMC Biology

Fig. 1

From: Aneuploidy-induced proteotoxic stress can be effectively tolerated without dosage compensation, genetic mutations, or stress responses

Fig. 1

Robust growth and enhanced stress tolerance indicate physiologic tolerance to aneuploidy. a Karyotypes of six aneuploid strains were determined by qPCR. Given chromosome copy numbers are quantified relative to their euploid (wild type, WT) levels and duplicated chromosomes are highlighted in red. Error bars indicate SD between the left chromosome arm and right arm of one biological replicate (see Methods). b Growth rates of yeast strains (mean of 3 biological replicates ± SEM) in liquid synthetic complete (SC) media at 25 °C. Growth rate was monitored over time by measuring optical density at the times indicated. c Average doubling times of indicated yeast strains plotted against the number of additional aneuploidy-associated duplicated open reading frames. d Proliferation of WT and aneuploid strains grown on SC the presence of tunicamycin (TM, 1 μg/mL), hygromycin B (HygB, 0.5 mg/mL), cadmium chloride (CdCl2, 50 μM), or cycloheximide (CHX, 0.2 μg/mL). Fivefold serial dilutions of indicated strains were spotted on SC medium with or without indicated treatments. Three independent experiments were performed, all yielding consistent observations. Representative plate scans are shown

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