Fig. 6

Populations cultured at different temperatures before being exposed to cold-shock show different susceptibility to this stressor. a Schematic of the experimental setup to study the effect of pre-cold-shock cultivation temperature. Animals were cultured at 20 °C until young adulthood and then transferred to 15 °C, 20 °C, or 25 °C for 3.5, 2.5, and 1.5 days, respectively. Cold-shock was then performed for 16 h, and rehabilitation was performed for 1 day at the pre-cold-shock temperature. b–d Average number of beads, average of mean bead size, and average inter-bead distance of PVD for populations that undergo cold-shock as described in a. The lines are the 25th percentile, mean, and 75th percentile. Whisker is the standard deviation. Statistical analysis was performed with one-way ANOVA followed by Tukey’s (d) or Steel-Dwass (b, c) tests for multiple comparison with equal or unequal variance assumptions, respectively, and significance level determined using Bonferroni correction (α = 0.016) for multiple feature comparisons. *P < 0.016, **P < 0.001, and ***P < 0.0001