Fig. 5
From: ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
ACBE-mediated base editing effects with different lengths of the linker peptides. a Schematic representation of ACBE, ACBE-0C, ACBE-20C, ACBE-16N, and ACBE-48 N architecture. The red square frame indicated the change of linkers. b Summary of flow cytometry results of HEK293-EGFP cells transfected ACBE variants with EGFP-Stop1 and EGFP-Stop2. c, d Summary of ACBE variants-mediated base editing patterns and efficiencies of all C and A in P53-G7 (c) and P53-G8 (d) targeting sites. e, f Summary of ACBE-mediated simbase editing patterns and efficiencies of all C and A in P53-G7 (e) and P53-G8 (f) sites using sgRNAs with different spacer lengths. The abovementioned values and error bars indicate mean ± s.e.m. of three independent experiments. The controls were untreated cell samples