Fig. 4
From: Putative ligand binding sites of two functionally characterized bark beetle odorant receptors
Functional characterization of ItypOR49. a Response of TREx/HEK293 cells expressing ItypOR49 and ItypOrco to select stimuli (30 μM) and vehicle control in the screening experiment (responses to all 68 odor stimuli are shown in Supplementary Fig. 3, Additional file 2). (+)-Induction: response of cells induced to express ItypOrco and ItypOR49; (−)-Induction: response of non-induced control cells. Asterisks (***) indicate a significant difference (at p < 0.001) between induced and non-induced cells; ns = not significant (n = 3 biological replicates, each including 3 technical replicates, i.e., ntotal = 9). b Dose-dependent responses of the same TREx/HEK293 cell line to the pure enantiomers of ipsdienol and the racemate (n = 6 biological replicates, each including 3 technical replicates, i.e., ntotal = 18). EC50 values: (R)-(−)-ipsdienol 9.47 μM; (±)-ipsdienol 5.34 μM. Data represent mean responses ± SEM