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Fig. 2 | BMC Biology

Fig. 2

From: Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-bet+ B cells

Fig. 2

Equine monocyte/DC major cell group is comprised of diverse cell types including a range of monocyte transcriptional states and distinct dendritic cell subtypes. a UMAP of monocyte/DC clusters with putative cluster annotations. Cluster 29 (annotated as neutrophils) was excluded from analysis due to low transcript (UMI) counts. b Frequency of each cell cluster within the monocyte/DC group per horse. c Hierarchical clustering (integrated PCA dimensions) indicates two subpopulations, putatively annotated as monocytes (clusters 18, 11, 12, 28) and DCs (19, 24, 26). d Heatmap of differentially expressed genes (adjusted p value < 0.05, log2 fold-change > 1 for each cluster versus all other clusters, expressed > 25% of cluster) by cluster. e Dot plot of select differentially expressed genes across monocyte clusters. Dot size is proportional to number of cells with detectable expression of indicated gene. Dot color intensity indicates average gene expression values scaled across plotted clusters. *Gene ID ENSECAG00000006663 is labeled FCGR3A/B based on Ensembl/NCBI annotations. f Dot plot of select differentially expressed genes across DC clusters. *Gene ID ENSECAG00000035431 is labeled SIRPA based on Ensembl/NCBI annotations. Additional details as in e. g Hierarchical clustering of equine PBMC scRNA-Seq data (monocyte/DC clusters) and human PBMC scRNA-Seq data (monocyte/DC clusters). Median-normalized average expression values for highly variable human/horse one-to-one orthologs were calculated for each cluster, and clustering was performed on Pearson distances by Ward’s method

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