Fig. 6

The CD3⁺PRF1⁻ clusters include naïve CD4⁺ and CD8⁺ T cells and additional CD4⁺ T cell populations. Clusters within the CD3⁺PRF1⁻ lymphocyte major cell group were further analyzed and annotated by differential gene expression. a UMAP subset of CD3⁺PRF1⁻ lymphocyte clusters (left) and of re-clustering analysis with putative cluster annotations (right). Selected axis ranges excluded < 10 cells in CD3⁺PRF1⁻ group from the UMAP subset plot. b Frequency of each cell cluster within the CD3⁺PRF1⁻ lymphocyte group per horse. c Expression patterns for genes characteristic of CD4/CD8 T cell subsets, naïve T cell populations (CCR7, SELL, LEF1), and a distinct CD3⁺CD4⁻CD8⁻CD200⁺ lymphocyte population (CD200, UBA2C). Expression values are scaled independently for each plot, ranging from 2.5 to 97.5 percentile of gene expression across all CD3⁺PRF1⁻ cells. Gene ID ENSECAG00000000775 labeled as CD8B based on Ensembl/NCBI annotation