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Fig. 4 | BMC Biology

Fig. 4

From: Functional analysis of sense organ specification in the Tribolium castaneum larva reveals divergent mechanisms in insects

Fig. 4

Comparison of the expression patterns of pan-neural genes. Light micrographs of flat preparations stained with DIG labelled RNA probe of Tc ase, Tc pros, and Tc sna. Open arrowheads: tracheal pits. a Tc ase is strongly expressed in the developing brain, the antennae, and the VNE (asterisk). Arrows: scattered Tc ase+ cells in the remaining appendages (arrows) and the lateral body wall (large arrowhead). Small arrowheads: plTSOs. b Small arrowheads: Tc pros expression in plTSOs. Large arrowheads: Tc pros expression dorsal to the tracheal pits in alBSMs. Tc pros is also strongly expressed in the developing brain, in clusters of cells in all appendages (arrows) and the VNE (asterisk). c Similarly, Tc sna is expressed in the brain, clusters of cells in the appendages (arrows) and VNE (asterisk). Large arrowheads: Tc sna is expressed in large clusters in the lateral body wall. The expression extending below the tracheal pits might correspond to the developing plTSOs (small arrowheads). d At NS15.1, groups and single cells express Tc pros in the lateral body wall, which seem to cover all areas of ESO formation. Additionally, the medial row that does not give rise to ESOs expresses Tc pros. Due to their prominent positions relative to the tracheal pits, the alBSM clusters, the plCSM clusters (large arrowhead), and the three TSO clusters (small arrowheads; plTSO, pvTSO1–2) are easily identifiable. e Tc sna shows a transient expression pattern in groups and single cells, some of which cover the areas where sensilla appear next to landmarks, such as the plCSMs (small arrowheads) in the thoracic segments and the plTSOs in the abdominal segments (large arrowhead). For abbreviations, see Fig. 1. Scale bar in a, 50 μm in a–c; scale bar in d, 50 μm in d, e

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