Fig. 6

RNAi phenotypes of cuticles. Laser-scanning confocal images of L1 cuticles; anterior is to the left. a Sensilla analysed in RNAi experiments in a negative control cuticle. Please note that alBSM is not visible in a4 (empty turquoise circle). b On the head of the Tc ASH RNAi cuticle, only few CSGs and the ant_TSOs are present. c On t1–3 of Tc ASH RNAi cuticles, only pdCSG (t1), plCSMs and pdCSGs (t2 and t3) are present. The remaining CSMs and the two BSMs are missing. d On a1–8 of the Tc ASH RNAi cuticle, all three types of sensilla (BSMs, CSGs, and TSOs) are affected. e The Tc ato RNAi cuticle of the head shows missing ant_TSOs (yellow arrows). f, g The Tc poxn RNAi cuticle shows duplications of specific sensilla (plCSM, alBSM, and pvTSO1) on thorax and abdomen. f On the thorax, the plCSMs (dotted blue circle) are duplicated, and an additional sensillum is found between alBSM and plCSM on t2 and t3, which has the morphological characteristics of plCSMs (black arrows). g On a2–a8, pvTSO1s are duplicated in Tc poxn RNAi cuticles (dotted yellow circles). An additional sensillum is found posterior to the alBSMs in a1–6 (black arrows). The additional sensillum exhibits a longer shaft compared to the wildtype BSMs. h On the head of Tc ct RNAi cuticles, ant_TSOs (yellow arrows), CSMs and BSMs have shorter shafts (blue and turquoise circles). ver_tri1–2 are missing. i On t1, adCSM1, plCSM, and pdCSG develop a socket only. On t2, the alBSM is missing. j The shafts of the CSGs and alBSMs are shorter or only developed as sockets. plTSOs are missing; pvTSOs have shorter shafts. Asterisks in i and j indicate missing tracheal pits. Scale bar in a, 100 μm; scale bar in b, 50 μm