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Fig. 1 | BMC Biology

Fig. 1

From: Exacerbated age-related hearing loss in mice lacking the p43 mitochondrial T3 receptor

Fig. 1

Exacerbated age-related hearing and hair cell loss in p43−/− mice. a Age-related auditory brainstem response (ABR) thresholds. Note the high- to low-frequency gradient of the deterioration of ABR thresholds in both strains, but more prominent in p43−/− (red plot) than in WT (blue plot) mice. b Age-related distortion product otoacoustic emission (DPOAE) amplitudes. Note that the DPOAE amplitudes decrease with age in both strains, but more severe in p43−/− (red plot) than in WT (blue plot) mice. The gray dots and shaded area represent the background noise levels. Data are expressed as mean ± SD (1 month: n = 31; 6 months: n = 23; 12 months: n = 15 mice per strain). One-way ANOVA test followed by Dunn’s test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, p43−/− vs. WT mice). c e, g, i Representative scanning electron microscopy (SEM) micrographs showing the apical, mid, and basal cochlear regions from p43−/− (e, e, and g) and WT (i) at 1 (c), 6 (e), and 12 (g and i) months of age. d, f, h, j Higher magnification of SEM images of representative OHC and IHC stereocilia from p43−/− (d, f, and h) and WT (j) at 1 (d), 6 (f), and 12 (h and j) months of age. Note the fused stereocilia of IHCs in f, h, and j. Scale bars: c, e, g, and i = 25 μm; d, f, h, and j = 10 μm. OHCs, outer hair cells; IHCs, inner hair cells; white star indicating OHC loss; yellow arrows pinpointing stereocilial fusion. kn Cytocochleograms representing the percentage of missing OHCs (k) and IHCs (m), and fused OHCs (l) and IHCs (n) in apical, mid, and basal regions from the cochleae of p43−/− (red bars) and WT (blue bars) aged 1, 6, and 12 months. Data are expressed as mean ± SD (n = 6 to 12 mice per age and strain). One-way ANOVA test was followed by Dunn’s test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, p43−/− vs. WT mice of the same age. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, older p43−/− vs. 1-month-old p43−/−. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, older WT vs. 1-month-old WT

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