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Fig. 5 | BMC Biology

Fig. 5

From: Single-cell RNA-seq reveals a concomitant delay in differentiation and cell cycle of aged hematopoietic stem cells

Fig. 5

HSPC differentiation trajectory associates with transcriptional programs that are altered upon aging. a, b Heatmaps showing standardized regulon activity scores, recovered with the AUCell procedure of Scenic, for young (left panel) and aged (right panel) HSPCs across Monocle states. Cells (columns) were ordered according to their pseudotime, and color bars at the top of the heatmaps indicate the state at which cell belongs (1 gray, 2 yellow, 3 green, 4 orange, and 5 blue). Regulons (rows) were hierarchically clustered, based on their activity score in young HSPCs. In a, 4 clusters of regulons are highlighted when analyzing regulon activity along pseudotime trajectories from s to L fate and from s to pMye bifurcation point (i.e., across Monocle states 1, 2 and 1, 3). In b, regulon activity along pseudotime trajectories from s to NeuMast and from s to MkEr fates (i.e., across states 1, 3, 4 and 1, 3, 5) is analyzed and 4 other clusters of regulons are highlighted. Arrows mark regulons for which a significant difference of activity with aging (average AUCell score difference between young and aged cells > 0.002 and p value < 0.05) were found in at least one of the considered states (i.e., states 1, 2, and 3 in a and 1, 3, 4, and 5 in b). The color indicates if regulon activity is increased (purple) or decreased (orange) in aged condition

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