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Fig. 3 | BMC Biology

Fig. 3

From: Toxic stress-specific cytoprotective responses regulate learned behavioral decisions in C. elegans

Fig. 3

ccBA, but not ccDA, activates specific systemic cytoprotective responses. a Representative epifluorescent microscopic images of DAF-16::GFP nuclear translocation in response to a 50-min 35 °C heat stress (HS) or a 30-min exposure to 1 μl ccBA or 4 μl ccDA in young adults. b Representative epifluorescent microscopic images of SKN-1::GFP nuclear translocation in response to 4 mM paraquat (PQ) for 1 h, 1 μl ccBA or 4 μl ccDA for 30 min in L3 larvae. Please note the specific fluorescence of the larger nuclei labeled by arrows in the image and in the inset of some samples and the granular intestinal autofluorescence present in each sample. c Representative epifluorescent microscopic images of cyp-35B1p::gfp expression in response to a 4-h exposure of 1 μl ccBA or 4 μl ccDA in worms fed by control empty vector (EV) and daf-16 RNAi. d Representative epifluorescent microscopic images of gst-4::gfp expression in response to a 4-h exposure of 1 μl ccBA or 4 μl ccDA in nematodes fed by EV and skn-1 RNAi. e Quantification of cyp-35B1p::gfp expression in response to a 4-h exposure to ccBA or ccDA in worms fed by control EV (top row of panel c). f Quantification of gst-4::gfp expression in response to a 4-h exposure to ccBA or ccDA in nematodes fed by control EV (top row of panel d). Please note the lack of detectable fluorescent signal in nematodes fed by daf-16 or skn-1 RNAi. Data are expressed as mean ± SEM. N, number of independent experiments; n, number of animals. p values were obtained by one-way ANOVA with Fisher’s LSD post hoc test (e) or unpaired Student’s t test following the evaluation of normal distribution significance by the Kolmogorov-Smirnov test (f). The inter-experimental variation (%CV) for (f) was 20% (control), 22% (ccBA), and 25% (ccDA). n.s., not significant; ***p < 0.001

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