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Fig. 1 | BMC Biology

Fig. 1

From: A brain cyst load-associated antigen is a Toxoplasma gondii biomarker for serodetection of persistent parasites and chronic infection

Fig. 1

Depletion of MORC phenocopies the inhibition of HDAC3 by inducing the expression of bradyzoite stage-specific genes. a Venn diagram illustrating the overlap between the MORC-regulated genes in RH and in Pru strains, and the 331 genes whose expression were reported to be exclusive to bradyzoite. b Expression of protein upon MORC depletion in both type I (RHku80) and type II (Pruku80) intracellular tachyzoites. Samples were taken 24 h after the addition of IAA and were probed with the sera from mice during acute (5 days) or chronic (> 5 weeks) toxoplasmosis. The experiment was repeated three times, and representative blots are shown. c Heat map showing the clustering analysis of selected bradyzoite genes and their corresponding proteins that are differentially regulated after the depletion of MORC for different combinations of strain and induction. Parasites were left unstimulated (US) or treated with FR235222 (FR) to inhibit HDAC3. The abundance of their transcripts in the various stages of development, namely early and mature bradyzoites, enteroepithelial stages (EESs), or three developmental stages of oocysts, is shown. The color scale indicates log2-transformed fold changes. d Chart illustration showing the disorder score as a function of protein amino acid position (generated via the IUPred server). The C-terminal domain end of BCLA (residues 1089 to 1275, hereafter referred to as rBCLA) is predicted as structured as opposed to the rest of the protein-containing core repeated motifs (in green). e Chart illustration showing the structural motifs of Toxoplasma gondii (TG) and Neospora caninum (NC) BCLA proteins. f Alignment of the 13 central repeats of the BCLA protein of the ME49 strain. Peptides 1 and 2 have served to direct antibodies against BCLA in rabbits. g Alignment of repeats of the BCLA protein of Neospora caninum which is characterized by two distinct families each comprising 10 repeated elements

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