Skip to main content
Fig. 2 | BMC Biology

Fig. 2

From: The CRK2-CYC13 complex functions as an S-phase cyclin-dependent kinase to promote DNA replication in Trypanosoma brucei

Fig. 2

Knockdown of CYC13 and CRK2 by tetracycline-inducible RNAi caused defective S-phase progression. a Western blotting to detect CYC13-PTP and CRK2-3HA in their respective RNAi cells. TbPSA6 served as the loading control. b Cell growth curves of the CYC13 RNAi cell line and the CRK2 RNAi cell line. ***p < 0.001 (chi-square test). c. Effect of CYC13 and CRK2 depletion on cell cycle progression. Cells with different numbers of nucleus (N) and kinetoplast (K) before and after RNAi induction were counted. A total of 100 cells were counted for each time point, and error bars indicated S.D. from three independent experiments (n = 3). d Flow cytometry analysis of CYC13 RNAi cells and CRK2 RNAi cells. Insets show the percentage of cells at different cell cycle stages. e EdU incorporation assay in non-induced control, CYC13 RNAi, and CRK2 RNAi cells. Scale bar: 5 μm. f Quantitation of EdU-positive 1N1K cells in non-induced control, CYC13 RNAi-induced cells, and CRK2 RNAi-induced cells. Error bars indicate S.D. from three independent experiments (n = 3). ***p < 0.001 (chi-square test)

Back to article page