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Fig. 3 | BMC Biology

Fig. 3

From: The CRK2-CYC13 complex functions as an S-phase cyclin-dependent kinase to promote DNA replication in Trypanosoma brucei

Fig. 3

Knockdown of CYC13 and CRK2 by tetracycline-inducible RNAi increased S-phase cells. a KKT13 as an S-phase marker in T. brucei. Shown is the localization of KKT13, which was tagged with a triple HA epitope from its endogenous locus in the CYC13 RNAi cell line and the CRK2 RNAi cell line, in non-induced control cells. Scale bar: 5 μm. b KKT13 expression in CYC13 RNAi-induced and CRK2 RNAi-induced cells. KKT13 was detected with FITC-conjugated anti-HA antibody. Scale bar: 5 μm. c Quantitation of KKT13-positive 1N1K cells before and after CYC13 RNAi and CRK2 RNAi induction. A total of 100 cells were counted for each cell line and each time point, and error bars indicated S.D. from three independent experiments (n = 3). ***p < 0.001 (chi-square test). d KKT13-3HA was detected in the two nuclei of CYC13-depleted bi-nucleated (2N1K and 2N2K) cells. Scale bar: 5 μm. e Quantitation of CYC13-positive bi-nucleate cells in non-induced control and CYC13 RNAi-induced cells. Error bars indicate S.D. from three independent experiments (n = 3)

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