Fig. 2

Immunostaining of acetylated tubulin. a Representative immunostaining image of KM fibers in an unwounded cell. b Representative immunostaining images of KM fibers in cells wounded in Regime 1. Red lines outline the possible location of a wound. The dashed blue line in the healed cell at 10 min indicates the possible location of folding, as seen by the discontinuity in the KM fibers on either side of this line (blue arrows). c Representative immunostaining images of KM fibers in cells wounded in Regime 2. All fluorescence images were acquired on the inverted confocal microscope. All scale bars 100 μm