Table 1 Comparison of typical key aspects of slow cooling and vitrification
Feature | Slow cooling | Vitrification |
|---|---|---|
Duration | Long (h) | Short (s) |
Usage of CPAs | Low concentrations | High concentrations |
Costly components | Equipment | Operation |
Controllability/reproducibility/recordability | High | Low |
Operation skills required | Low | High |
Main risks | Extracellular ice formation, osmotic damage | Intracellular ice formation, CPA toxicity, fracturing, osmotic stress |