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Fig. 7 | BMC Biology

Fig. 7

From: Loss of splicing factor IK impairs normal skeletal muscle development

Fig. 7

Myoblast proliferation and apoptosis was not affected in ik KO embryos. a Comparison of cell proliferation estimated through BrdU incorporation in 3 dpf WT and ik KO embryos. The relative number of BrdU-positive cells shown in the graph. ns not significant. b Analysis of apoptosis in 3 and 5 dpf WT and ik KO live embryos with acridine orange staining. c Immunoblot analysis of PARP, cleaved caspase-3, and cleaved caspase-9 in C2C12 myoblasts transfected with siIK#1 for 48 h

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