Skip to main content
Fig. 2 | BMC Biology

Fig. 2

From: H3K27 modifiers regulate lifespan in C. elegans in a context-dependent manner

Fig. 2

Pathway analysis of mes-2, jmjd-3.2 and utx-1 mediated lifespan extension. mes-2, jmjd-3.2 and utx-1 mutant alleles were subjected to daf-16 RNAi and lifespan assays performed in order to investigate possible lifespan-extending pathways. ac RNAi depletion of daf-16 in mes-2(tm5007) (a), jmjd-3.2(tm3121) (b) and utx-1(tm3118)/+ (c) backgrounds completely suppresses lifespan extension. d Combinatorial knockdown of jmjd-3.2 (using mutant allele tm3121) and utx-1 (using post-embryonic RNAi) results in increased life extension compared to either the jmjd-3.2 allele (**p = 0.007) or utx-1 RNAi alone (*p = 0.03). Controls are shared between experiments a, b and c (as the experiments were performed as a large set) although the graphs are separated for clarity. EV, empty vector control (i.e. worms fed HT115 bacteria transformed with L4440 RNAi vector lacking a genomic insert) (see Additional file 7: Table S4 for the full statistical analysis of data, including repeats)

Back to article page