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Fig. 4. | BMC Biology

Fig. 4.

From: BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells

Fig. 4.

G4 flow cytometry analysis of human PBMC cells. a Histogram plot of the BG4 signal distribution in human PBMC incubated with BG4 (black) or without antibody (white). b Labeling of PBMC cells from a healthy donor with the BG4 antibody (white). Nucleus border is defined by the white line. Scale bar 10 μm. c Quantification of the BG4 signal in human PBMC cells from an AML patient or a healthy donor. Plotted results were based on the average of n = 3 technical independent experiments processed at three different time points. Significance was calculated based on a one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype cells: **p < 0.01. d Histogram plot of the BG4 signal distribution in human PBMC extract from an AML patient (gray) or a healthy donor (black). An excel file providing individual data values is available in the section “Availability of data and materials”

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