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Fig. 4 | BMC Biology

Fig. 4

From: Long-term persistence of supernumerary B chromosomes in multiple species of Astyanax fish

Fig. 4

Astyanax B-linked genes sharing and B chromosome emergence. a Examples of the two coverage patterns found. Regions amplified by qPCR are shaded in gray. UC = uniform coverage and IC = irregular coverage. b Venn diagram showing the B-linked genes shared by the four Astyanax species analyzed here. Names in bold refer to genes with uniform coverage in the B chromosome of A. scabripinnis. Note that the approach used in this study cannot find unique genes in the lineages of A. bockmanni and A fasciatus. c Circos plot showing the location of the paralogs of B-linked genes on the regular chromosomes of A. mexicanus. Note that some validated genes were unplaced in the A. mexicanus genome. Names in bold refer to genes with uniform coverage in the B chromosome of A. scabripinnis and the numbers within parenthesis indicate the number of species where B chromosomes harbors the gene paralog. d Phylogram built with the full mitogenomes of all samples analyzed here. Green circles indicate the nodes used to calibrate the molecular clock. The most relevant result is that the node separating the four species carrying large metacentric B chromosomes is 3.75 mya. Intervals in node dates represent the 95% confidence interval. * = Bayesian posterior probability higher than 0.9

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