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Fig. 1 | BMC Biology

Fig. 1

From: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles

Fig. 1

MODIP screening for production of DI244. MDCK-PB2(sus) cells were cultivated in shake flask (50 mL working volume) in Xeno™ medium. Cells were infected with a purely clonal DI244 seed virus in exponential growth phase at a VCC of 2E+6 cells/mL at MODIPs ranging from 1E−1 to 1E−4. Samples (supernatants) were analyzed for a HA titer, b DI244 titer (plaque assay in MDCK-PB2(adh) cells), c VCC, and d vRNA level of DI244, Seg5, and Seg8 (real-time RT-qPCR). Results show a single set of experiments; additional independent experiments performed for subsequent investigations (the “Interfering efficacy of DI244 material strongly depends on the MODIP used for production,” “SXC purification results in an increased interfering efficacy,” and “DI244 material shows antiviral effect in the mouse model” sections) showed comparable results (Additional file 1: Table S1)

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