Fig. 3From: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particlesInterfering efficacy of DI244 material produced at different MODIPs. For the interference assay, parental adherent MDCK cells were infected with STV at MOIs of 10, or 0.01 and co-infected with DI244 material (125 μL), produced at a MODIP ranging from 1E−1 to 1E−4, or medium as negative control (NC). The supernatant was sampled 16 hpi (STV MOI 10) or 24 hpi (STV MOI 0.01). a Infectious virus titers were quantified by plaque assay (parental adherent MDCK cell). The total amount of virus particles was determined by hemagglutination assay. b vRNA of DI244, Seg5, and Seg8 were investigated using real-time RT-qPCR. The interference assay was performed in independent experiments (n = 3) using one DIP preparation for each MODIP; error bars indicate standard deviationBack to article page