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Fig. 3 | BMC Biology

Fig. 3

From: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles

Fig. 3

Interfering efficacy of DI244 material produced at different MODIPs. For the interference assay, parental adherent MDCK cells were infected with STV at MOIs of 10, or 0.01 and co-infected with DI244 material (125 μL), produced at a MODIP ranging from 1E−1 to 1E−4, or medium as negative control (NC). The supernatant was sampled 16 hpi (STV MOI 10) or 24 hpi (STV MOI 0.01). a Infectious virus titers were quantified by plaque assay (parental adherent MDCK cell). The total amount of virus particles was determined by hemagglutination assay. b vRNA of DI244, Seg5, and Seg8 were investigated using real-time RT-qPCR. The interference assay was performed in independent experiments (n = 3) using one DIP preparation for each MODIP; error bars indicate standard deviation

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