Fig. 5.
Getting accurate cell morphology measurements in non-flat samples with DeProj. a 3D visualization of the NSC population on the zebrafish telencephalon generated by DeProj. The cells are drawn with their approximate contour, their color encoding the number of neighbor cells (from dark blue to yellow: 2 to 16 neighbors). b Cell apical area, measured on the 3D surface, a3D. c Cell apical area measured on the 2D projection, a2D. The color scale is identical in b and c. d Histogram of the error metric on area (1−a2D/a3D) for all the cells of the epithelial-like surface. e Rendering of this error on the epithelial-like surface. f Rendering of the slope of the apical plane of each cell with the XY plane. g Correlation between the slope of the apical plane and the error on cell area for all cells of the epithelial-like surface. Red line: 100×(1−cosθ)