Fig. 7
Antennal lobe (AL) glomeruli after larval RNAiOrco. Representative single optical slices of the AL of freshly eclosed (A0) beetles of the EF-1-B line (A, A’, B, B’) and 7-day-old beetles (A7) of the Orco-GAL4 line (C, C’, D, D’) after dsRNAOrco injection in late larvae with comparison to wildtype beetles visualized by phalloidin (A–D; green) and synapsin (A’–D’; magenta). A, B In freshly eclosed beetles, the AL glomeruli are clearly visible in the phalloidin and synapsin staining under both conditions. c, d At A7 glomeruli are clearly visible in both stainings in untreated beetles, while glomeruli in knockdown beetles are hardly visible in the phalloidin staining, and only vague glomeruli are visible in the synapsin staining. Scale bars 20 μm