Fig. 1From: Single-cell spatial transcriptomic analysis reveals common and divergent features of developing postnatal granule cerebellar cells and medulloblastomaSingle-cell transcriptome profiling of postnatal cerebellar cells. a Workflow (top panel) for cerebellum collection by FACS-sorted, single-cell sequencing, and analysis of two samples [one at postnatal days 7 (P7) and the other at P11] from Math1-GFP mice and two samples [one at P7 and the other at P11] from Dcx-DsRed mice. Workflow (bottom panel) for cerebellum section, spatial transcriptomes, and analysis of two samples from WT mice (both at P7). b Strains were established from transgenic Math1-GFP and transgenic Dcx-DsRed mice. Scale bar: 100 μm. c t-SNE visualization of 24,919 cells from FACS-sorted samples (n = 4; Math1-GFP mice at P7 and P11; Dcx-DsRed mice at P7 and P11). Cells are colored according to clusters with annotation of cell types. d Dot plot for the expression of marker genes in each cell type. Color represents the mean expression in each cell cluster, and size indicates the fraction of cells expressing marker genes. e t-SNE visualization of 24,919 cells from FACS-sorted samples: Math1-GFP+ and Dcx-DsRed+ samples. f Bar plot showing the proportion of cell types in Math1-GFP+ and Dcx-DsRed+ samples. P < 0.0001. P values were determined using Pearson’s chi-square testBack to article page