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Fig. 3. | BMC Biology

Fig. 3.

From: A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography

Fig. 3.

Overview image, processing for ribbon detection, section recognition, and focus map acquisition. a 20x LM overview image, b Preprocessed ribbons in LM (see also supplementary Figure 1 for individual steps). c Segmented sections; overlaid onto the overview image. df Close-up of the first section as indicated by the magenta rectangle. g Example focus map showing manual focus positions (+). These can be updated during the complete process if needed. The color code is indicating the interpolated focus z values. Scale bar in ac = 500 μm. Scale bar in df = 50 μm

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