Fig. 4

Cell type-specific APA events across GABAergic neurons. a Schematic overview of the machine learning-based method in SAPAS used to identify cell type-specific APA events. b The histogram depicts the distribution of AUROCs of all genes on classifying PVBC neurons from other GABAergic neuron types. The Y-axis represents the counts, and the X-axis represents the AUROC. c The t-SNE plot of six different GABAergic neuron types on gene expression level. The neuron types are labeled using different colors as shown. d The t-SNE plots depict the signal distributions of the poly(A) site usage of identified cell type-specific APA events for different GABAergic neuron types (Calm1 for PVBC, Kif3c for MNC, Map4 for LPC, Pcmt1 for ISC, Ube2d3 for CHC, and Naa50 for CCKC). The blue gradient represents the poly(A) site usage as indicated. e Synaptic communication-related GO terms (biological process) enriched in genes with cell type-specific APA events for each GABAergic neuron type. The normalized enrichment scores (NES) calculated by GSEA are shown by red gradient, and the P-values are shown by circle size