Fig. 1

Characterization of cExNPCs and cINPCs from patient-derived iPSCs. a Differentiation schemes for generating cExNPCs and cINPCs, including timeline, small molecules used, and maintenance as a suspended or adherent embryoid body (EB/aEB). b CHRNA7 expression in cExNPCs and cINPCs was analyzed by RT-qPCR. Data is the average +/-SEM from four independent biological replicate experiments (n = 4). c cExNPCs were seeded in equal numbers for each line tested and counted after four days of maintenance (n = 7 biological replicates). d cExNPCs were stained with propium iodide for DNA content and analyzed by FACS. S and 4N (M phase) cells were quantified for each study subject. Average values are shown from seven independent biological replicates (n = 7). e cINPCs were seeded in equal numbers for each line tested and counted after 4 days of maintenance (n = 7 biological replicates). f cINPCs were stained with propidium iodide for DNA content and analyzed by FACS. S and 4N (M phase) cells were quantified for each model. Values are from seven independent biological replicate experiments (n = 7). All significant findings in this manuscript were confirmed in three or more independent biological replicate experiments performed using two clonal lines for the UM and AP models, and one clonal line for the UC-F and UC-M models, as summarized in Additional file 4. Clones used, replicates, and data values are in Additional file 4. Plot shows the median value, calculated by using a Kruskal-Wallis non-parametric test as described in the Methods. P values: *P < 0.05, **P < 0.01, and ***P < 0.001