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Fig. 2 | BMC Biology

Fig. 2

From: Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7

Fig. 2

Generation and characterization of cortical neuroids. a Schematic of method used to generate cortical neuroids, by combining cExNPCs and cINPCs (1:1 ratio) and differentiating and maturing them for 15 days. ABC=ascorbic acid, BDNF, and cAMP. See the “Methods” section for further details. bd Immunocytochemistry with antibodies indicated detects cINPCs (DLX2), proliferating NPCs (Ki67), and cExNPCs (TBR1 and PAX6), with representative images from one clonal line per subject shown. e RNA-seq analysis defined differences in gene expression between the AP, UM, and UC-M neuroids for the markers shown (n = 4 independent biological replicates from one clonal line per subject). f Immunocytochemical quantification of the percentage of cells expressing the proliferative marker Ki67, n = 4 biological replicate experiments utilizing two clonal lines for the AP and UM and one clonal line for the UC-M. Clones used, replicates, and data values are in Additional file 4. Scale bars=50μm

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