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Fig. 3 | BMC Biology

Fig. 3

From: Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7

Fig. 3

Morphometric analysis of differentiated cortical neuroids. ac Five days after plating cortical neuroids in differentiation media, neurite length was analyzed for each sample type. a Representative light microscopy images are shown. Quantification was performed as described in the “Methods” section, by defining the distance between the border of the plated neuroid and the tips of neurites extending from that neuroid on a per-EB basis. Scale bar=250 μm. b Immunocytochemical analysis of neurite extension using MAP2 staining, with representative images shown. Scale bar=150 μm. c Quantification of neurite extension is shown for seven biological replicate experiments (n = 7). Plot shows the median value, calculated by using Kruskal-Wallis non-parametric tests, as described in the Methods. (d-d’) Neurite length was analyzed in plated MAP2 immunostained neurons, using data from three independent biological replicate experiments (n = 3). (e-e”) Expression of the GABA and glutamate transporters, VGAT and VGLUT, was assessed by immunocytochemical analysis of these neurons. Representative images are shown in e and synaptic puntae were quantified in (e’-e”) for VGAT (e’) and VGLUT (e”). Data were derived by quantifying ~15 stained neuroids derived from four independent biological replicate experiments (n = 4). Clones used, replicates, and data values are in Additional file 4. Scale bar=75 μm. Plot shows the median value, calculated by using Kruskal-Wallis non-parametric tests as described in the “Methods” section. P values: *P < 0.05, **P < 0.01, and ***P < 0.001

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