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Fig. 3 | BMC Biology

Fig. 3

From: Age-related susceptibility to insulin resistance arises from a combination of CPT1B decline and lipid overload

Fig. 3

A HFD increases mitochondrial capacity to oxidize fatty acids only in young mice. a Total PGC1α protein content in quadriceps homogenates normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels. b Citrate synthase activities measured in quadriceps homogenates and mitochondria-enriched suspensions.  Mitochondrial O2 consumption fluxes were corrected for mitochondria enrichment of suspensions using the ratio between citrate synthase activity (CS) in the mitochondria vs in total homogenate (R = CSmito/CShomog). Maximal ADP-stimulated O2 consumption per tissue protein is shown for palmitoyl-CoA and carnitine (c), pyruvate (d) and pyruvate and glutamate (e) as substrates, all in the presence of malate. Data are shown as mean ± SEM and were analysed using two-way ANOVA. Multiple comparisons were performed using Tukey’s correction, n = 4–6 (young groups) or 8–10 (old groups); **p < 0.01 (HFD vs LFD), *p < 0.05 (HFD vs LFD), matched ages. Supporting data values can be found in Additional file 10

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