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Fig. 7 | BMC Biology

Fig. 7

From: Pathologic tau conformer ensembles induce dynamic, liquid-liquid phase separation events at the nuclear envelope

Fig. 7

Interference with nuclear trafficking. a Normal nuclear-cytoplasmic compartmentalization (NCC) observed in both 4RD-YFP tau reporter (Ctrl) and ES1 cells transiently transfected with the NCC reporter. Scale bars, 20 μm and 10 μm in the boxed images. b The mean pixel intensity of the red fluorescent signals (left) and the coefficient of variation (CV) (right) in the transfected reporter (n = 8) and ES1 cells (n = 8) shown in a. RFP, red fluorescent protein; a.u., arbitrary unit. c Time-lapse imaging of FRAP analysis. Red fluorescence signals (colored in magenta) were completely photobleached and then images of signal recovery were obtained every 10 min for 6 h. The arrowhead in magenta indicates the point of photobleaching applied. Scale bar, 10 μm. d Real-time measurements of fluorescence recovery of nuclear red signals in tau reporter (n = 8) and ES1 cells (n = 8) shown in a. The means of individual time points were compared to one another. *p < 0.05 by one-way ANOVA with Tukey’s multiple comparison test. e The signal recoveries in the last five time points revealed a significant decline in the nuclear trafficking of RFP in ES1 cells compared to the controls. Error bars represent SEM. ***p < 0.001 in comparison with the controls. f The variation in the CV of the mean pixel intensities measured in the FRAP analysis from d

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