Fig. 8

Nuclear deformation in FTLD-MAPT patients. a Distortion of neuronal nuclear envelopes was determined in the dentate gyrus of FTLD-MAPT patients using lamin B1 staining for nuclear lamina (upper panels). Ctrl, non-neurological disease. Lower panels: higher magnification views to show representative nuclear morphologies including Cleft, nuclear cleft; Cytopl, cytoplasmic granular lamin B stain; Discont, discontinuous nuclear edge; Angular, angled nuclear envelope. b Assessment of nuclear deformation in FTLD-MAPT (FTLD) (n = 10 subjects), non-neurological disease (controls, Ctrl) (n = 5), and other neurodegenerative diseases (OND) (n = 10) including Alzheimer’s disease (AD), frontotemporal dementia with progranulin mutations (GRN), amyotrophic lateral sclerosis (ALS), and amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD). Lesions of the dentate gyrus obtained from 20 to 30 field images were scored on 3-point scale by two observers (see also “Methods” and Table 1). p-tau (AT8), phosphorylated tau detected using the monoclonal antibody, AT8. *p < 0.05 and ***p < 0.001 by one-way ANOVA with Tukey’s multiple comparison test. Scale bars, 40 μm and 20 μm in the boxed images